Configure, install, and operate your blitz system and blitz pro software. It also provides documented verification that the octet instrument operates as intended at the user site by inspecting the critical components. First read the medication guide that comes inside your forteo carton. Hudson robotics lab automation fortebio octet workcell. Data kindly provided by maxplanckinstitute of experimental medicine in gottingen, germany see jahn et al. Octet red384 getting started introduction load analyte sample. Operation of fortebio molecular interactions instrument the fortebio red octet 96 instrument uses biolayer interferometry to measure binding of molecues to a protein immobilized on a probe. From chemistry analyzers that analyze urine, blood, and plasma, to bioanalyzers that use microfluidic technology. Although the measuring the amount of bound material is done in a different way from surface plasmon resonance spr in the well known biacore instruments.
This spr analyzer has capabilities ranging from proteinprotein down to proteinsmall molecule and has a rapid sampling rate. Exploring blocking assays using octet, proteon, and. Proteon xpr36 equipped with glc sensor chips and coupling reagents 10 mm sodium acetate ph 4. Fremont, ca prweb april 05, 2017 pall fortebio, the market leader in labelfree biomolecular interaction analysis across academic research, drug discovery and development, process analytics, and manufacturing quality control today announced the launch of the new octet red96e instrument. Mar 03, 2015 fortebio octet qk labelfree analyzer with computer and octet software lot 400. The latest release, version 11, is available for use on all octet systems. This spr analyzer has capabilities ranging from proteinprotein down to proteinsmall molecule and has a rapid sampling rate that enables fast and slow kinetics with low noise. Biomolecular binding kinetics assays on the octet platform 14 introduction direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Identification of a common epitope between enterovirus 71. Epitope binning by fortebio octet platform creative biolabs. Fortebio octet qk labelfree analyzer with computer and octet.
Since its sensors are disposable and do not involve the handling of liquid samples, the prospect of cross contamination is virtually eliminated, thereby enabling parallel antibody screening applications. Utilizes biolayer interferometry bli to enable label free, realtime analysis of protein. From kinetics and quantitation analysis of antibodies, proteins, protein residual contaminants and other biological molecules, octet software is intuitive and easy to use. Pall fortebio, a leading supplier of instrumentation for accelerated drug discovery and development, has announced the launch of the octet k2 system, a new entry point into the octet line of labelfree interaction analysis. Octet realtime drug and protein binding kinetics unit. The htx read head provides design flexibility that allows users to analyze, 8, 16, 32, 48, or 96. The octet red384 is an instrument for biolayer interferometry bli, an optical. Identification of a common epitope between enterovirus 71 and. Increasing access to sensitive binding interactions analysis.
Experience the identifiably different spectramax id3 multimode microplate reader. Octet qke system pall fortebio 47661 fremont boulevard fremont, ca 94538 t 888. The octet htx and octet red384 utilize fortebios dip and read technology with the read head dipping into samples in a plate format. Uom description 305048 system system includes octet red96 instrument, octet software v6. Do not store your forteo pen with the needle attached. The octet red96e system detects a diverse range of bio molecules from small. In addition, the sample material remains available after analysis for further investigation. Serial fivefold concentrations of med25 were analyzed, and each of the concentrations yielded the same results kd 8. The analysis software needs baseline, association and dissociation steps consecutively to work. Pall fortebio launches the octet k2 system 7aug2015.
Octet red96egxp system included octet red96e instrument, octet cfr software, desktop computer, lc monitor, accessory kit, iqoq kit, pq kits and oneyear warranty 1852 pack singleuse evaporation covers to extend the experiment up to 12 hours. The versatile 8channel octet systems provide flexibility for rapid assay development. In addition, installation and qualification iqoq and performance qualification pq kits are available for gxp users. Pall fortebio octet systems european biotechnology. Converting an elisa assay into an octet quantitation assay. Table of recommened sensor offsets by well volume from octet user manual. The sample compartment of biacore t200 can be cooled down to 4c, enabling the analysis of sensitive samples in unattended runs of up to 48 h. If you do not follow these instructions carefully, you may get the wrong forteo dose. For more details, please check the data analysis user guide provided by octet qk system. Providing technologies to accelerate therapeutics development. Efficiently integrating pcr into laboratory processes can be challenging, and in many cases manual thermocycling has created a bottleneck that has increased demands on laboratory. Servswitch octet kv1701, servswitch octet kv1702, servswitch octet kv1703, servswitch octet. Both automated and manual ingel digest protocols need approximately one hour of preparation green bar, but automation frees the user from additonal handson time red bars over a seven hour period.
Fortebio acquisition by sartorius molecular devices. Remember to put into the concentration of mab and antigen. The blitz system and blitz pro software user guide contains information on materials. Click here for more information on purchasing assay kits outside of the united states. For questions or to get started, please contact unit director alexey tomilov, phd, at. The fortebio octet red system gives users labelfree interaction detection, rapid data acquisition, and high sensitivity. The octet htx and octet red384 utilize fortebio s dip and read technology with the read head dipping into samples in a plate format. Fortebio introduces the octet system technology networks.
The iqoq kit provides comprehensive documentation to verify the octet instrument, accessories and computer system are supplied and installed per fortebios guidelines by certified personnel. Epitope binning by fortebio octet platform when multiple antibodies have similar affinities for a target, determining whether they bind to the same or different epitopes on the target molecule becomes an inevitable step towards evaluating their individual functional potential. Contact information the fortebio octet red384 system is located in 3006 veterinary medicine research facility 3b vm3b. Biolayer interferometry bli using the octet red96e system. Lab analyzers can be used to analyze samples in a variety of settings.
On april 30, 2020, sartorius ag acquired life science company fortebio, the former biologics business unit of molecular devices. High throughput octet htx and octet red384 systems fortebio. For information on preparing samples for quantitation or kinetics experiments, please see the appropriate fortebio octet biosensor product instructions. Biomolecular binding kinetics assays on the octet platform 2 of lead candidates during development. The use of all four flow cells allows four interactions to be simultaneously monitored.
Additionally, we determined the affinity of 2h2 binding to med25 using the fortebio octet system, which applies the optical interference theory and produces highly accurate and reproducible data. As an increasing number of research methods currently require a polymerase chain reaction pcr step, the thermocycler has become an essential tool in the modern life science laboratory. Accordingly, downstream processes require detailed characterization of specificity, selectivity, stability and binding rate analyses under various growth or buffer conditions to facilitate informed process decisions. Pall fortebio launches the octet red96e system for. Forteo fortayo teriparatide injection user manual important. Servswitch octet kv1700 switch pdf manual download. Operation of fortebio molecular interactions instrument. Using the more complex models available in the octet analysis software can help troubleshoot more complex curves and optimize the design of kinetics experiments fortebio offers different chemistry biosensors for off theshelf use octet can use up to 16 biosensors for scouting experiments, and each sensor is independent. Responsible for designing, developing and executing the test specification and test plans for octet pro 5. General requirement seeking contractor to provide all labor, tools, material, parts, equipment, supervision, software, license, certification, transportation, management, and personnel required for a preventive maintenance on government equipment octet red384 system, located in bldg.
Summar y octet systems provide an automated solution for maximizing the efficiency of antibody detection and quantitation in biopharmaceutical development and manufacturing processes. Octet red384 getting started introduction the octet red384 is an instrument for biolayer interferometry bli, an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. Follow the directions carefully when using the forteo delivery device. A food safety tester or pathogen analyzer or clinical analyzer. Servswitch octet kv1701, servswitch octet kv1702, servswitch octet kv1703, servswitch octet kv1712, servswitch octet kv17, servswitch octet kv1711. Protein detection and analysis tecan life sciences home. Before you use your new forteo delivery device, please read the entire front and back of this user manual completely. For each experiment, youll need to bring fortebio biosensors, at least 2 black. Labelfree analytical technologies such as the octet platform from pall fortebio provide a powerful means to obtain accurate information. Includes computer equipped with octet user software 4. Keeping the needle on may let forteo leak from the pen, and air bubbles can form in the cartridge.
Black box servswitch octet kv1700 user manual pdf download. Fortebio octet qk labelfree analyzer with computer and. Welcome to the fortebio octet system data acquisition user guide. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput based on need. The instrument should be on for at least 1 hour prior to running an experiment. The 8channel octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1. Lab analyzers can provide info on the size, the quantity of cells and the purity of samples. The octet software kinetics analysis and reporting user software enabling graph exporting in a number of different formats streamlined data analysis and model fitting within origin 7. These comparison tables provide a general guide to the features included in various versions of the software. Biacore t200 supports the use of 96 and 384well microplates and vials. With no fluidics involved, octet systems are highly robust. The system has been developed to operate reliably in a regulated environment.
View and download black box servswitch octet kv1700 user manual online. The octet qke has provided us with a robust and versatile platform for the measurement of antibody antigen interactions. The octet red96e system is designed to work reliably in regulated environments. Significantly easier, faster and better characterization of drug candidates is possible, providing greater value in drug development applications where existing methods have limitations in throughput, performance and cost. Developing a robust quantitation assay for monoclonal. Blitz user guide introduction welcome to the fortebio blitz user guide. Fortebio octet red96 analysis of macromolecular interactions and binding kinetics data acquisition manual data analysis manual sample preparation. Fortebio octet qk labelfree analyzer with computer and octet software lot 400. Octet red384 getting started introduction the octet red384 is an instrument for biolayer interferometry bli, an optical technique for. Instrument qualification the iqoq validation manual and testing kit provides a stepbystep procedure for the installation qualification iq and operation qualification oq of the blitz system. Octet is designed to accelerate antibody development by generating realtime quantitative and kinetics results quickly and easily. Fortebio octet data acquisition and data analysis software is rapidly evolving to better address the growing applications and needs of our customers.
Controlled access with multiple user privileges adminis trator, developer. Octet htx system for high throughput detection and fine resolution analysis for quantitation and kinetic characterization, using labelfree, realtime analysis. Fortebio acquired by sartorius ag apr 30, 2020 on april 30, 2020, sartorius ag acquired life science company fortebio, the former biologics business unit of molecular devices. Octet systems enable realtime, labelfree analysis for determination of affinity, kinetics and concentration. Both are performed on a solid support on which the capture molecule is immobilized and the analyte is bound from solution.437 479 1481 699 783 915 1217 120 1457 463 1201 1208 1452 234 601 49 998 1046 315 205 645 1491 1207 191 358 683 1406 555 998 93 1109 59 636 1490 50 1473 1435